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pentr directional topo cloning kit  (Thermo Fisher)


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    Thermo Fisher pentr directional topo cloning kit
    Pentr Directional Topo Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+directional+topo+cloning+kit/pentr+d+topo+cloning+kit/us12203927-255-15-20
    Average 90 stars, based on 1 article reviews
    pentr directional topo cloning kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sequencing:

    Article Title: Role of α2δ-3 in regulating calcium channel localization at presynaptic active zones during homeostatic plasticity
    Article Snippet: .. The α2δ-3 coding sequence was cloned from UAS-stj-3HA (FlyORF F001252) transgenic flies and inserted into the pENTR-D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen K240020). .. Mutations were introduced in the MIDAS motif ( D G S G S to A G A G A , UAS-α2δ-3 DSS-AAA ) or RLR site ( RLR to AAA , UAS-α2δ-3 RLR-AAA ) of α2δ-3 using the Q5 site-directed mutagenesis kit (NEB E0554S).

    Clone Assay:

    Article Title: Role of α2δ-3 in regulating calcium channel localization at presynaptic active zones during homeostatic plasticity
    Article Snippet: .. The α2δ-3 coding sequence was cloned from UAS-stj-3HA (FlyORF F001252) transgenic flies and inserted into the pENTR-D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen K240020). .. Mutations were introduced in the MIDAS motif ( D G S G S to A G A G A , UAS-α2δ-3 DSS-AAA ) or RLR site ( RLR to AAA , UAS-α2δ-3 RLR-AAA ) of α2δ-3 using the Q5 site-directed mutagenesis kit (NEB E0554S).

    Article Title: Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
    Article Snippet: .. Resulting PCR products were cloned using a pENTR Directional TOPO Cloning kit (Thermo Fisher Scientific) following manufacturers protocols. .. An expression construct was generated by performing an LR recombination reaction (Thermo Fisher Scientific) between the entry clone and the Gateway destination vector pVHD, a Vanillate-inducible gene expression plasmid for Sphingomonas (pVHD was a gift from Julia Vorholt; Addgene plasmid # 61303; http://n2t.net/addgene:61303 ; RRID:Addgene_61303).

    Article Title: Characterisation of fatty acyl reductases of sunflower (Helianthus annuus L.) seed.
    Article Snippet: .. The amplified fragments were cloned into the pENTR/D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen). .. The products were then transferred to the pK7FWG2 vector (Karimi et al., 2002, 2007) using the GATEWAY recombination cloning system (ThermoFisher Scientific, USA) at the attL x attR (LR) recombination sites.

    Article Title: GATA transcription factor in common bean: A comprehensive genome-wide functional characterization, identification, and abiotic stress response evaluation
    Article Snippet: .. The PvGATA28 was cloned into the plant expression vector using the pENTR Directional TOPO Cloning Kit from Invitrogen (UK). ..

    Article Title: OsCRY2 and OsFBO10 co-regulate photomorphogenesis and photoperiodic flowering in indica rice.
    Article Snippet: Cryptochromes (CRYs) are a class of photoreceptors that perceive blue/ultraviolet-A light of the visible spectrum to mediate a vast number of physiological responses in bacteria, fungi, animals and plants.. In the present study, we have characterized OsCRY2 in a photoperiod sensitive indica variety, Basmati 370, by generating and analyzing overexpression (OE) and knock-down (KD) transgenic lines.. The OsCRY2OE lines displayed dwarfism as shown in their reduced plant height and leaf length, attributed largely by an overall reduction in their cell size.

    Transgenic Assay:

    Article Title: Role of α2δ-3 in regulating calcium channel localization at presynaptic active zones during homeostatic plasticity
    Article Snippet: .. The α2δ-3 coding sequence was cloned from UAS-stj-3HA (FlyORF F001252) transgenic flies and inserted into the pENTR-D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen K240020). .. Mutations were introduced in the MIDAS motif ( D G S G S to A G A G A , UAS-α2δ-3 DSS-AAA ) or RLR site ( RLR to AAA , UAS-α2δ-3 RLR-AAA ) of α2δ-3 using the Q5 site-directed mutagenesis kit (NEB E0554S).

    Cloning:

    Article Title: Role of α2δ-3 in regulating calcium channel localization at presynaptic active zones during homeostatic plasticity
    Article Snippet: .. The α2δ-3 coding sequence was cloned from UAS-stj-3HA (FlyORF F001252) transgenic flies and inserted into the pENTR-D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen K240020). .. Mutations were introduced in the MIDAS motif ( D G S G S to A G A G A , UAS-α2δ-3 DSS-AAA ) or RLR site ( RLR to AAA , UAS-α2δ-3 RLR-AAA ) of α2δ-3 using the Q5 site-directed mutagenesis kit (NEB E0554S).

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 μL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https:// www.thermofisher.com/order/catalog/product/K240020 ). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Article Title: Methods for immunoregulation by modulating plasminogen-apple-nematode (PAN) domain-containing proteins
    Article Snippet: .. The pENTR/D-TOPO entry vector was used for the insertion of the PCR products using the pENTR Directional Topo Cloning Kit (Thermo Fisher Scientific). .. LR Clonase II Enzyme Mix (Thermo Fisher Scientific) was used to transfer the coding sequences into the pGWB402-omega destination vector and transformed into One Shot TOP10 Chemically Competent cells.

    Article Title: Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
    Article Snippet: .. Resulting PCR products were cloned using a pENTR Directional TOPO Cloning kit (Thermo Fisher Scientific) following manufacturers protocols. .. An expression construct was generated by performing an LR recombination reaction (Thermo Fisher Scientific) between the entry clone and the Gateway destination vector pVHD, a Vanillate-inducible gene expression plasmid for Sphingomonas (pVHD was a gift from Julia Vorholt; Addgene plasmid # 61303; http://n2t.net/addgene:61303 ; RRID:Addgene_61303).

    Article Title: Characterisation of fatty acyl reductases of sunflower (Helianthus annuus L.) seed.
    Article Snippet: .. The amplified fragments were cloned into the pENTR/D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen). .. The products were then transferred to the pK7FWG2 vector (Karimi et al., 2002, 2007) using the GATEWAY recombination cloning system (ThermoFisher Scientific, USA) at the attL x attR (LR) recombination sites.

    Article Title: GATA transcription factor in common bean: A comprehensive genome-wide functional characterization, identification, and abiotic stress response evaluation
    Article Snippet: .. The PvGATA28 was cloned into the plant expression vector using the pENTR Directional TOPO Cloning Kit from Invitrogen (UK). ..

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells.
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 mL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https://www.thermofisher. com/order/catalog/product/K240020). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Article Title: OsCRY2 and OsFBO10 co-regulate photomorphogenesis and photoperiodic flowering in indica rice.
    Article Snippet: Cryptochromes (CRYs) are a class of photoreceptors that perceive blue/ultraviolet-A light of the visible spectrum to mediate a vast number of physiological responses in bacteria, fungi, animals and plants.. In the present study, we have characterized OsCRY2 in a photoperiod sensitive indica variety, Basmati 370, by generating and analyzing overexpression (OE) and knock-down (KD) transgenic lines.. The OsCRY2OE lines displayed dwarfism as shown in their reduced plant height and leaf length, attributed largely by an overall reduction in their cell size.

    Purification:

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 μL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https:// www.thermofisher.com/order/catalog/product/K240020 ). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells.
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 mL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https://www.thermofisher. com/order/catalog/product/K240020). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Polymerase Chain Reaction:

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 μL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https:// www.thermofisher.com/order/catalog/product/K240020 ). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Article Title: Methods for immunoregulation by modulating plasminogen-apple-nematode (PAN) domain-containing proteins
    Article Snippet: .. The pENTR/D-TOPO entry vector was used for the insertion of the PCR products using the pENTR Directional Topo Cloning Kit (Thermo Fisher Scientific). .. LR Clonase II Enzyme Mix (Thermo Fisher Scientific) was used to transfer the coding sequences into the pGWB402-omega destination vector and transformed into One Shot TOP10 Chemically Competent cells.

    Article Title: Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
    Article Snippet: .. Resulting PCR products were cloned using a pENTR Directional TOPO Cloning kit (Thermo Fisher Scientific) following manufacturers protocols. .. An expression construct was generated by performing an LR recombination reaction (Thermo Fisher Scientific) between the entry clone and the Gateway destination vector pVHD, a Vanillate-inducible gene expression plasmid for Sphingomonas (pVHD was a gift from Julia Vorholt; Addgene plasmid # 61303; http://n2t.net/addgene:61303 ; RRID:Addgene_61303).

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells.
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 mL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https://www.thermofisher. com/order/catalog/product/K240020). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Plasmid Preparation:

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 μL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https:// www.thermofisher.com/order/catalog/product/K240020 ). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Article Title: Methods for immunoregulation by modulating plasminogen-apple-nematode (PAN) domain-containing proteins
    Article Snippet: .. The pENTR/D-TOPO entry vector was used for the insertion of the PCR products using the pENTR Directional Topo Cloning Kit (Thermo Fisher Scientific). .. LR Clonase II Enzyme Mix (Thermo Fisher Scientific) was used to transfer the coding sequences into the pGWB402-omega destination vector and transformed into One Shot TOP10 Chemically Competent cells.

    Article Title: Characterisation of fatty acyl reductases of sunflower (Helianthus annuus L.) seed.
    Article Snippet: .. The amplified fragments were cloned into the pENTR/D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen). .. The products were then transferred to the pK7FWG2 vector (Karimi et al., 2002, 2007) using the GATEWAY recombination cloning system (ThermoFisher Scientific, USA) at the attL x attR (LR) recombination sites.

    Article Title: Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cells.
    Article Snippet: .. Clone resulting purified PCR products (5–10 ng of fresh PCR product in a final volume of 0.5–4 mL) using a pENTR Directional TOPO cloning kit (Thermo Fisher Scientific) in Escherichia coli TOP10 competent cells following manufacturer protocols Thermo Fisher (https://www.thermofisher. com/order/catalog/product/K240020). pENTR/D-TOPO plasmid includes a cassette for kanamycin resistance. a. ..

    Article Title: OsCRY2 and OsFBO10 co-regulate photomorphogenesis and photoperiodic flowering in indica rice.
    Article Snippet: Cryptochromes (CRYs) are a class of photoreceptors that perceive blue/ultraviolet-A light of the visible spectrum to mediate a vast number of physiological responses in bacteria, fungi, animals and plants.. In the present study, we have characterized OsCRY2 in a photoperiod sensitive indica variety, Basmati 370, by generating and analyzing overexpression (OE) and knock-down (KD) transgenic lines.. The OsCRY2OE lines displayed dwarfism as shown in their reduced plant height and leaf length, attributed largely by an overall reduction in their cell size.

    Amplification:

    Article Title: Characterisation of fatty acyl reductases of sunflower (Helianthus annuus L.) seed.
    Article Snippet: .. The amplified fragments were cloned into the pENTR/D-TOPO vector using the pENTR Directional TOPO Cloning Kit (Invitrogen). .. The products were then transferred to the pK7FWG2 vector (Karimi et al., 2002, 2007) using the GATEWAY recombination cloning system (ThermoFisher Scientific, USA) at the attL x attR (LR) recombination sites.

    Article Title: OsCRY2 and OsFBO10 co-regulate photomorphogenesis and photoperiodic flowering in indica rice.
    Article Snippet: Cryptochromes (CRYs) are a class of photoreceptors that perceive blue/ultraviolet-A light of the visible spectrum to mediate a vast number of physiological responses in bacteria, fungi, animals and plants.. In the present study, we have characterized OsCRY2 in a photoperiod sensitive indica variety, Basmati 370, by generating and analyzing overexpression (OE) and knock-down (KD) transgenic lines.. The OsCRY2OE lines displayed dwarfism as shown in their reduced plant height and leaf length, attributed largely by an overall reduction in their cell size.

    Expressing:

    Article Title: GATA transcription factor in common bean: A comprehensive genome-wide functional characterization, identification, and abiotic stress response evaluation
    Article Snippet: .. The PvGATA28 was cloned into the plant expression vector using the pENTR Directional TOPO Cloning Kit from Invitrogen (UK). ..



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